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	Provedor de dados Electron. J. Biotechnol. (3) BABT (2) Anais da ABC (AABC) (1) BJMBR (1) Autor Aiguang,Guo (1) Astray,Renato Mancini (1) Asurmendi,Sebastián (1) Barrera Avellaneda,Luis A (1) Bazzini,Ariel A (1) COSTA,FABRÍCIO F. (1) CUNHA,NICOLAU B. (1) Chen,Fang (1) Costa,Bruno Labate Vale da (1) Costa,F.F. (1) DIAS,SIMONI C. (1) Echeverri Peña,Olga Yaneth (1) FRANCO,OCTÁVIO L. (1) Fangli,Wu (1) Gonçalves,M.S. (1) Hopp,H. Esteban (1) Hu,Xiaole (1) Jin,Liu (1) Jorge,Soraia Attie Calil (1) Junqueira,M.L. (1) Mais... Palavra-chave Transient expression (7) Antimicrobial peptides (1) BHK-21 (1) E. coli (1) Endosperm-specific (1) Fetal hemoglobin (1) GFP (1) Gene silencing (1) Glutenin (1) Glycation (1) HC-Pro (1) HPFH (1) Hereditary persistence of fetal hemoglobin (1) Heterologous expression (1) Human sulfatase (1) Large-scale bioprocesses (1) Magnifection (1) Mammalian cells (1) Nicotiana benthamiana (1) PDS (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Journal article (3) Info:eu-repo/semantics/other (1) Ano 2019 (1) 2014 (1) 2013 (1) 2010 (1) 2009 (1) 2007 (1) 2001 (1) Mais... País Brazil (4) Chile (3) Idioma Inglês (7)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 7 Primeira ... 1 ... Última A novel chloroplastic isopentenyl diphosphate isomerase gene from Jatropha curcas: Cloning, characterization and subcellular localization Electron. J. Biotechnol. Wei,Lei; Yin,Li; Hu,Xiaole; Xu,Ying; Chen,Fang. Background Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic and molluscicidal. But not much is known about the pathway involved in the biosynthesis of terpenoids. The present investigation describes the cloning, characterization and subcellular localization of isopentenyl diphosphate isomerase (IPI) gene from J. curcas. IPI is one of the rate limiting enzymes in the biosynthesis of terpenoids, catalyzing the crucial interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Results A full-length JcIPI cDNA consisting of 1355 bp was cloned.... Tipo: Journal article Palavras-chave: Heterologous expression; Southern blot; Terpenoids; Transient expression. Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600007 A practical approach to the understanding and teaching of RNA silencing in plants Electron. J. Biotechnol. Bazzini,Ariel A; Mongelli,Vanesa C; Hopp,H. Esteban; del Vas,Mariana; Asurmendi,Sebastián. Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach... Tipo: Journal article Palavras-chave: Gene silencing; GFP; HC-Pro; PDS; Protocols; PTGS; Teaching; Transgenic; Transient expression; TRV; VIGS. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002 Cloning of high molecular weight gluten subunit promoter and study on its function in wheat BABT Weibo,Jin; Jin,Liu; Fangli,Wu; Aiguang,Guo. The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds. Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Glutenin; Promoter; Endosperm-specific; Transient expression. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002 Human sulfatase transiently and functionally active expressed in E. coli K12 Electron. J. Biotechnol. Poutou-Piñales,Raúl A; Vanegas Niño,Adriana; Landázuri,Patricia; Sáenz,Homero; Lareo,Leonardo; Echeverri Peña,Olga Yaneth; Barrera Avellaneda,Luis A. The recombinant human iduronate 2-sulfate sulfatase (hrIDS) was transiently and functionally active expressed in E. coli K12. The enzyme activity (crude extract) at 100 ml and 400 ml oscillated between 0.25 and 10.58 nmol h-1 mg-1. The wide Western-blot peptide profile suggest that hrIDS is proteolitically processed “randomly” which agrees with the ultrafiltration assay in which the hrIDS activity was found in all fractions (<30kDa, 30-100kDa and >100kDa). No glycation sites were found by computer analysis of the hIDS sequence; discarding the possibility of marks for glycation and proteolytic processing. Tipo: Journal article Palavras-chave: E. coli; Glycation; Human sulfatase; Transient expression. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300005 Molecular farming of antimicrobial peptides: available platforms and strategies for improving protein biosynthesis using modified virus vectors Anais da ABC (AABC) LEITE,MICHEL L.; SAMPAIO,KAMILA B.; COSTA,FABRÍCIO F.; FRANCO,OCTÁVIO L.; DIAS,SIMONI C.; CUNHA,NICOLAU B.. Abstract The constant demand for new antibiotic drugs has driven efforts by the scientific community to prospect for peptides with a broad spectrum of action. In this context, antimicrobial peptides (AMPs) have acquired great scientific importance in recent years due to their ability to possess antimicrobial and immunomodulatory activity. In the last two decades, plants have attracted the interest of the scientific community and industry as regards their potential as biofactories of heterologous proteins. One of the most promising approaches is the use of viral vectors to maximize the transient expression of drugs in the leaves of the plant Nicotiana benthamiana. Recently, the MagnifectionTM expression system was launched. This sophisticated commercial... Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial peptides; Magnifection; Nicotiana benthamiana; Transient expression. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000200851 Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production BABT Núñez,Eutimio Gustavo Fernández; Jorge,Soraia Attie Calil; Astray,Renato Mancini; Rezende,Alexandre Gonçalves de; Costa,Bruno Labate Vale da; Monteiro,Daniella Cristina Ventini; Pereira,Carlos Augusto; Tonso,Aldo. The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale. Tipo: Info:eu-repo/semantics/article Palavras-chave: BHK-21; Large-scale bioprocesses; Mammalian cells; Recombinant proteins; SFV; Transient expression. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500018 The Agamma-195 (C->G) mutation in hereditary persistence of fetal hemoglobin is not associated with activation of a reporter gene in vitro BJMBR Schreiber,R.; Gonçalves,M.S.; Junqueira,M.L.; Saad,S.T.O.; Krieger,J.E.; Costa,F.F.. Hereditary persistence of fetal hemoglobin is an uncommon, benign disorder in which the expression of gamma-globin genes persists into adult life. Several point mutations have been associated with the increased gamma-globin gene promoter activity. We evaluated the -195 (C->G) mutation by a functional in vitro assay based on the luciferase reporter gene system. The results indicated that the increased promoter activity observed in vivo could not be reproduced in vitro under the conditions employed, suggesting that other factors may be involved in the overexpression of the gamma-globin gene containing the -195 (C->G) mutation. Furthermore, this is the first time that the -195 (C->G) mutation of the Agamma-globin gene has been evaluated by in vitro... Tipo: Info:eu-repo/semantics/other Palavras-chave: Fetal hemoglobin; Hereditary persistence of fetal hemoglobin; HPFH; Transient expression. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400008 Registros recuperados: 7 Primeira ... 1 ... Última

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